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A new firefly luciferase with bimodal spectrum: Identification of structural determinants of spectral pH-sensitivity in firefly luciferases
(2005-07-01)
Fireflies emit flashes in the green-yellow region of the spectrum for the purpose of sexual attraction. The bioluminescence color is determined by the luciferases. It is well known that the in vitro bioluminescence color ...
A new firefly luciferase with bimodal spectrum: Identification of structural determinants of spectral pH-sensitivity in firefly luciferases
(2005-07-01)
Fireflies emit flashes in the green-yellow region of the spectrum for the purpose of sexual attraction. The bioluminescence color is determined by the luciferases. It is well known that the in vitro bioluminescence color ...
Estudo comparativo do efeito da temperatura na estabilidade e espectros de bioluminescência das luciferases de Phrixotrix hirtus e outros besouros bioluminescentes
(Universidade Federal de São CarlosUFSCarPrograma de Pós-Graduação em Genética Evolutiva e Biologia Molecular - PPGGEvCâmpus São Carlos, 2019-03-22)
Firefly luciferases have been extensively used as bioanalytical reagents and their cDNAs as reporter genes and bioimaging biosensors. Luciferases of fireflies, “rail-road” worm and "tec-tec" beetles, have different ...
Clonagem e caracterização das isoformas de luciferases de lanternas e corpo gorduroso do vagalume Aspisoma lineatum (Lampyridae: Cratomorphini): origem estrutural e evolutiva das luciferases pH-sensitivas mais eficientes à partir de ancestrais pH-insensitivas
(Universidade Federal de São CarlosUFSCarPrograma de Pós-Graduação em Genética Evolutiva e Biologia Molecular - PPGGEvCâmpus São Carlos, 2020-10-31)
Firefly luciferases emit yellow-green light with high efficiency at physiological pH. However, under acidic conditions, in the presence of heavy metals or high temperatures, they emit red light. To understand the structural ...
The influence of the loop between residues 223-235 in beetle luciferase bioluminescence spectra: A solvent gate for the active site of pH-sensitive luciferases
(2008-01-01)
Beetle luciferases emit a wide range of bioluminescence colors, ranging from green to red. Firefly luciferases can shift the spectrum to red in response to pH and temperature changes, whereas click beetle and railroadworm ...
The influence of the loop between residues 223-235 in beetle luciferase bioluminescence spectra: A solvent gate for the active site of pH-sensitive luciferases
(2008-01-01)
Beetle luciferases emit a wide range of bioluminescence colors, ranging from green to red. Firefly luciferases can shift the spectrum to red in response to pH and temperature changes, whereas click beetle and railroadworm ...
The structural origin and biological function of pH-sensitivity in firefly luciferases
(2008-02-19)
Firefly luciferases are called pH-sensitive because their bioluminescence spectra display a typical red-shift at acidic pH, higher temperatures, and in the presence of heavy metal cations, whereas other beetle luciferases ...
The structural origin and biological function of pH-sensitivity in firefly luciferases
(2008-02-19)
Firefly luciferases are called pH-sensitive because their bioluminescence spectra display a typical red-shift at acidic pH, higher temperatures, and in the presence of heavy metal cations, whereas other beetle luciferases ...
Pyrearinus termitilluminans larval click beetle luciferase: Active site properties, structure and function relationships and comparison with other beetle luciferases
(2009-12-01)
Several beetle luciferases have been cloned and sequenced. However, most studies on structure and function relationships and bioanalytical applications were done with firefly luciferases, which are pH sensitive. Several ...
Pyrearinus termitilluminans larval click beetle luciferase: Active site properties, structure and function relationships and comparison with other beetle luciferases
(2009-12-01)
Several beetle luciferases have been cloned and sequenced. However, most studies on structure and function relationships and bioanalytical applications were done with firefly luciferases, which are pH sensitive. Several ...